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壮医针挑疗法对哮喘小鼠miRNA-126/TSLP的影响
蓝凰齐,王有科,唐汉庆
0
(广西中医药大学,广西 南宁,530001;右江民族医学院,广西 百色,533000)
摘要:
目的:观察壮医针挑疗法对哮喘小鼠miRNA-126/TSLP的影响。方法:选取6~8周龄的SPF级BALB/c小鼠,将其随机分为对照组、模型组、针挑组、阳性组、阴性组,每组各10只。对除对照组外的其余组小鼠采用卵清蛋白致敏及激发方法制备哮喘模型,针挑组采用壮医针挑疗法,阳性组给予miRNA-126抑制剂,阴性组给予miRNA-126激动剂,每天1次,连续干预7d。干预后对各组小鼠进行肺功能(FEV0.1、FEV0.2、FEV0.4/FVC、PEF)测定。眼球取血,采用ELISA法检测IL-4、IL-5、IL-10、IL-13水平;取外周血,采用RT-PCR法检测miRNA-126、TSLP基因的相对表达。结果:与对照组比较,模型组、阴性组肺功能各项指标均降低,IL-4、IL-5、IL-10、IL-13水平及miRNA-126、TSLP基因相对表达均升高,差异均有统计学意义(P<0.05或P<0.01);与模型组比较,针挑组、阳性组肺功能各项指标均升高,IL-4、IL-5、IL-10、IL-13水平以及miRNA-126、TSLP基因相对表达均下降,差异均有统计学意义(P<0.05或P<0.01)。结论:针挑疗法治疗哮喘、改善肺功能的起效环节与其调节miRNA-126/TSLP基因表达、降低Th2型细胞因子水平有关。
关键词:  哮喘  壮医针挑疗法  miRNA-126  TSLP  实验研究
DOI:
Effect of needle-picking therapy of Zhuang medicine on miRNA-126/TSLP in mice with asthma
LAN Huangqi,WANG Youke,TANG Hanqing
(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;Youjiang Medical University for Nationalities,Bose 533000,Guangxi,China)
Abstract:
Objective:To investigate the effect of needle-picking therapy of Zhuang medicine on miRNA-126/TSLP in mice with asthma.Methods:Specific pathogen-free BALB/c mice,aged 6-8 weeks,were randomly divided into control group,model group,needle-picking group,positive group,and negative group,with 10 mice in each group.All mice except those in the control group were treated with ovalbumin sensitization and challenge to establish a model of asthma;the mice in the needle-picking group were given needle-picking therapy of Zhuang medicine,those in the positive group were given miRNA-126 inhibitor,and those in the negative group were given miRNA-126 agonist,once a day for 7 consecutive days.Pulmonary function parameters (forced expiratory volume in 0.1 second,forced expiratory volume in 0.2 second,forced expiratory volume in 0.4 second/forced vital capacity,and peak expiratory flow) were measured after intervention.Blood samples were collected from the eyeball and ELISA was used to measure the levels of interleukin-4 (IL-4),interleukin-5 (IL-5),interleukin-10 (IL-10),and interleukin-13 (IL-13);peripheral blood samples were collected and RT-PCR was used to measure the relative mRNA expression of miRNA-126 and TSLP.Results:Compared with the control group,the model group and the negative group had significant reductions in the above pulmonary function parameters and significant increases in the levels of IL-4,IL-5,IL-10,and IL-13 and the relative mRNA expression of miRNA-126 and TSLP (P<0.05 or P<0.01).Compared with the model group,the needle-picking group and the positive group had significant increases in these pulmonary function parameters and significant reductions in the levels of IL-4,IL-5,IL-10,and IL-13 and the relative mRNA expression of miRNA-126 and TSLP (P<0.05 or P<0.01).Conclusion:The needle-picking therapy can treat asthma and improve pulmonary function by regulating the mRNA expression of miRNA-126/TSLP and reducing the levels of Th2 cytokines.
Key words:  asthma  needle-picking therapy  miRNA-126  TSLP  experimental study

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