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补益营卫方对衰老表皮结构蛋白K17的影响
焦翠琴,王 浩,周小平
0
(宁夏医科大学中医学院,宁夏 银川,750004)
摘要:
目的:观察补益营卫方对衰老表皮结构蛋白K17的基因和蛋白水平的影响。方法:将3个月龄小鼠(年轻组)与14个月龄小鼠(老年组)表皮细胞消化分离出来,然后进行传代培养,其中14个月龄小鼠(老年组)的表皮细胞分为2组,一组用常规方法培养细胞,另一组用含2.5%的补益营卫方培养基进行培养,采用荧光定量RT-PCR法和Western Blotting法分别检测细胞K17mRNA表达和细胞K17蛋白表达。结果:与年轻组比较,老年组的K17mRNA和蛋白表达水平均升高(P<0.05);经药物干预后小鼠表皮细胞中K17mRNA和蛋白水平较老年组明显降低(P<0.05)。结论:补益营卫方可通过抑制衰老表皮结构蛋白K17的表达,起到延缓皮肤衰老作用。
关键词:  衰老  补益营卫方  表皮结构  角蛋白17  实验研究
DOI:
Effect of Buyi Yingwei prescription on keratin 17 in senescent epidermal structure
JIAO Cuiqin,WANG Hao,ZHOU Xiaoping
(School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,Ningxia,China)
Abstract:
Objective:To investigate the effect of Buyi Yingwei prescription on the mRNA and protein expression of keratin 17 (K17) in senescent epidermal structure.Methods:Epidermal cells were isolated from mice aged 3 months (young group) and those aged 14 months (elderly group),and then subculture was performed.The epidermal cells from the elderly group were divided into two groups;one group was cultured with conventional method,and the other group was cultured in the medium containing 2.5% Buyi Yingwei prescription.RT-qPCR and Western blotting were used to measure the mRNA and protein expression of K17 in cells.Results:Compared with the young group,the elderly group had significant increases in the mRNA and protein expression of K17 (P<0.05).In the elderly group,the cells with drug intervention had significant reductions in the mRNA and protein expression of K17 compared with those without intervention (P<0.05).Conclusion:Buyi Yingwei prescription delays skin aging by inhibiting the expression of K17 in senescent epidermal structure.
Key words:  senescence  Buyi Yingwei prescription  epidermal structure  keratin 17  experimental study

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