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千杯少颗粒解酒作用机制研究
刘瑞连,李 鑫,曾普华
0
(湖南省中医药研究院,湖南 长沙,410006;湖南中医药大学,湖南 长沙,410208)
摘要:
目的:明确千杯少颗粒解酒作用机制。方法:取SPF级SD大鼠,按体质量分层随机分为空白组、模型组、阳性对照组(予海王金樽药液干预,给药剂量为0.81 g/kg)及千杯少颗粒低、中、高剂量组(予千杯少颗粒药液干预,给药剂量分别为8.1 g/kg、16.2 g/kg、32.4 g/kg)。除空白组外,各组均灌胃56°二锅头(10 ml/kg)制备醉酒模型,30 min后,给予相应药物干预,模型组及空白组灌胃等容积蒸馏水。给药1 h后将实验动物麻醉,腹主动脉采血,分离血清,测定乙醇含量及丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)水平。取肝组织,匀浆后,测定乙醇脱氢酶(ADH)与乙醛脱氢酶(ALDH)的活性。结果:与模型组比较,千杯少颗粒各剂量组均能不同程度地降低大鼠血清中乙醇含量及ALT、AST含量,提高大鼠肝组织中ADH、ALDH的活性,差异均有统计学意义(P<0.05或P<0.01)。结论:千杯少颗粒解酒作用机制可能与其增强肝组织中ADH、ALDH的活性及保护肝脏有关。
关键词:  千杯少颗粒  药效实验  作用机制  乙醇脱氢酶  乙醛脱氢酶
DOI:
Mechanism of the antialcoholic effect of Qianbeishao granules
LIU Ruilian,LI Xin,ZENG Puhua
(Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China;Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)
Abstract:
Objective:To investigate the mechanism of the antialcoholic effect of Qianbeishao granules.Methods:Specific pathogen-free Sprague-Dawley rats were stratified by body mass and were then randomly divided into blank group,model group,positive control group (treated with Haiwang Jinzun solution at a dose of 0.81 g/kg),and low-,middle-,and high-dose Qianbeishao granule groups (treated with the solution of Qianbeishao granules at a dose of 8.1 g/kg,16.2 g/kg,and 32.4 g/kg,respectively).All rats except those in the blank group were given Erguotou with an alcohol content of 56% vol (10 ml/kg) to establish a model of drunkenness,and corresponding drug intervention was given 30 minutes later;the rats in the model group and the blank group were given an equal volume of distilled water by gavage.The rats were anesthetized at 1 hour after administration,and blood samples were collected from the abdominal aorta and serum was obtained to measure the levels of alcohol,alanine aminotransferase (ALT),and aspartate aminotransferase (AST).Liver tissue was collected to prepare liver homogenate and measure the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).Results:Compared with the model group,the Qianbeishao granule groups had varying degrees of reductions in the serum levels of alcohol,ALT,and AST,as well as significant increases in the activities of ADH and ALDH in the liver (P<0.05 or P<0.01).Conclusion:Qianbeishao granules exert an antialcoholic effect by increasing the activities of ADH and ALDH in the liver and protecting the liver.
Key words:  Qianbeishao granule  pharmacological experiment  mechanism of action  alcohol dehydrogenase  aldehyde dehydrogenase

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