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基于网络药理学研究知柏地黄丸治疗特发性中枢性性早熟的活性成分及作用机制
李 博,廖艳红,何炜星
0
(湖南中医药大学第一附属医院,湖南 长沙,410007)
摘要:
目的:基于网络药理学研究知柏地黄丸治疗特发性中枢性性早熟(ICPP)的活性成分及作用机制。方法:通过TCMSP数据库筛选知柏地黄丸的有效成分,利用GeenCards、OMIM及MalaCards数据库收集ICPP的疾病靶点,使用韦恩图筛选疾病与有效成分的共同交叉靶点,利用Cytoscape 3.2.1构建知柏地黄丸-有效成分-交叉靶点-ICPP的网络药理学调控网络,并对知柏地黄丸核心成分进行网络拓扑分析,然后利用STRING对交叉靶点进行PPI网络构建,采用R包DOSE、clusterProfiler及pathview对交集靶点进行GO功能与KEGG通路富集分析。结果:筛选出知柏地黄丸潜在活性成分β-谷甾醇、常春藤皂苷元以及吴茱萸次碱等10个,核心靶点雌激素受体1(ESR1)、血管内皮生长因子A(VEGFA)、细胞色素P4503A4酶(CYP3A4)、雄激素受体(AR)、核受体亚家族3C组成员1(NR3C1)5个,核受体活性、配体激活的转录因子活性、类固醇受体活性等20个GO功能以及内分泌抵抗、雌激素信号通路、乳腺癌等6条KEGG通路。结论:知柏地黄丸通过多成分、多靶点、多通路、多途径的整体调控方式作用于ICPP发挥疗效,为其临床运用提供了科学依据。
关键词:  特发性中枢性性早熟  知柏地黄丸  网络药理学  活性成分  作用机制
DOI:
Mechanism of action of Zhibai Dihuang pills in treatment of idiopathic central precocious puberty based on network pharmacology
LI Bo,LIAO Yanhong,HE Weixing
(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China)
Abstract:
Objective:To investigate the mechanism of action of Zhibai Dihuang pills in the treatment of idiopathic central precocious puberty (ICPP) based on network pharmacology.Methods:TCMSP database was used to screen out the effective constituents of Zhibai Dihuang pills,and GeenCards,OMIM,and MalaCards databases were used to obtain the disease targets of ICPP.Venn diagram was used to screen out the intersecting targets of the disease and the effective constituents,and Cytoscape 3.2.1 was used to establish a network pharmacological regulatory network of Zhibai Dihuang pills-effective constituents-intersecting targets-ICPP and perform a network topology analysis of the core constituents of Zhibai Dihuang pills.STRING was used to construct a protein-protein interaction network of the intersecting targets,and R package DOSE,clusterProfiler,and pathview were used to perform gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the intersecting targets.Results:A total of 10 potential active constituents of Zhibai Dihuang pills were screened out,such as β-sitosterol,hederagenin,and rutaecarpin,and there were 5 core targets of Zhibai Dihuang pills,i.e.,estrogen receptor 1,vascular endothelial growth factor A,cytochrome P4503A4,androgen receptor,and nuclear receptor subfamily 3 group C member 1.There were 20 GO functions (including nuclear receptor activity,activity of ligand-activated transcription factors,and steroid receptor activity) and 6 KEGG pathways (including endocrine resistance,estrogen signaling pathway,and breast cancer).Conclusion:Zhibai Dihuang pills exert a therapeutic effect on ICPP via multiple constituents,targets,pathways,and channels,which provides a scientific basis for the clinical application of Zhibai Dihuang pills.
Key words:  idiopathic central precocious puberty  Zhibai Dihuang pills  network pharmacology  active constituent  mechanism of action

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